Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Grim19 CRISPR/Cas9 KO Plasmid (h): sc-403264
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Grim19 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Grim19 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Grim19 Antibody (F-10): sc-365978
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Grim19 CRISPR/Cas9 KO Plasmid (h) | sc-403264 | 20 µg | $397.00 |
Overview
NDUFA13 (GRIM19) encodes a conserved accessory subunit of mitochondrial complex I that supports oxidative phosphorylation and proper electron transfer within the respiratory chain. Grim19 contributes to maintenance of mitochondrial membrane potential and cellular bioenergetics, linking mitochondrial metabolism to redox homeostasis and apoptotic susceptibility. Beyond its role in complex I assembly and function, GRIM19 has been reported to interface with signal transduction programs such as STAT3-dependent transcription, providing a connection between mitochondrial status and nuclear gene regulation. Dysregulation of NDUFA13/Grim19 is associated with mitochondrial dysfunction phenotypes and has been studied in contexts where altered respiration and stress signaling contribute to disease-relevant cellular states.
Grim19 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NDUFA13 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NDUFA13 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NDUFA13 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Grim19 protein expression.
This CRISPR knockout system enables efficient generation of NDUFA13-deficient cell models for investigation of Grim19 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NDUFA13 exon(s) critical for Grim19 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NDUFA13 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Grim19 CRISPR/Cas9 KO Plasmid (h) and Grim19 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NDUFA13 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Grim19 HDR Plasmid (h) and Grim19 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NDUFA13 homology arms to support homology-directed repair at defined NDUFA13 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.