Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GRHL3 CRISPR/Cas9 KO Plasmid (h): sc-404946
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRHL3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GRHL3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GRHL3 Antibody (C-12): sc-398838
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRHL3 CRISPR/Cas9 KO Plasmid (h) | sc-404946 | 20 µg | $397.00 |
Overview
GRHL3 (grainyhead-like transcription factor 3) is a sequence-specific transcriptional regulator that coordinates epithelial differentiation, barrier formation, and wound-responsive gene programs. It helps maintain epidermal homeostasis by modulating keratinocyte proliferation and terminal differentiation networks and intersects with pathways controlling cell polarity, adhesion, and transcriptional control of structural and junctional genes. Dysregulated GRHL3 activity has been linked to developmental defects and epithelial pathologies, and altered expression patterns are reported in studies of squamous epithelia and carcinoma-associated transcriptional states. As a nuclear factor shaping epithelial gene expression, GRHL3 is frequently investigated in models of tissue morphogenesis, stress responses, and lineage commitment.
GRHL3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GRHL3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GRHL3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GRHL3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GRHL3 protein expression.
This CRISPR knockout system enables efficient generation of GRHL3-deficient cell models for investigation of GRHL3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GRHL3 exon(s) critical for GRHL3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GRHL3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GRHL3 CRISPR/Cas9 KO Plasmid (h) and GRHL3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GRHL3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GRHL3 HDR Plasmid (h) and GRHL3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GRHL3 homology arms to support homology-directed repair at defined GRHL3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.