GPR56 Double Nickase Plasmid (m): sc-420657-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR56 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR56 Double Nickase Plasmid (m) and GPR56 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Adgrg1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR56 Double Nickase Plasmid (m) | sc-420657-NIC | 20 µg | $410.00 | |||
GPR56 Double Nickase Plasmid (m2) | sc-420657-NIC-2 | 20 µg | $410.00 |
Adgrg1 encodes the adhesion G protein–coupled receptor GPR56 (ADGRG1), a membrane receptor that integrates extracellular matrix cues with intracellular signaling to regulate cell adhesion, migration, and polarity. GPR56 is processed into N- and C-terminal fragments and can couple to heterotrimeric G proteins, influencing pathways linked to cytoskeletal dynamics, mechanotransduction, and developmental patterning. In mouse systems, Adgrg1 is widely used to study neural and glial development as well as immune cell behavior, where receptor-mediated adhesion and motility are key determinants of tissue organization. Dysregulated GPR56 activity has been associated with neurodevelopmental and myelination-related phenotypes and is frequently examined in contexts where altered cell–matrix interactions contribute to disease-relevant biology.
GPR56 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Adgrg1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Adgrg1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Adgrg1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Adgrg1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.