GPR4 CRISPR/Cas9 KO Plasmid (h): sc-403659
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR4 CRISPR/Cas9 KO Plasmid (h) | sc-403659 | 20 µg | $397.00 |
Overview
GPR4 encodes a proton-sensing G protein-coupled receptor that functions as a cellular pH sensor, translating extracellular acidosis into intracellular signaling. Upon activation, GPR4 couples to heterotrimeric G proteins to modulate cAMP/PKA and phospholipase C–dependent pathways, influencing calcium flux, cytoskeletal dynamics, and transcriptional programs linked to endothelial and immune cell behavior. Its expression is enriched in vascular and inflammatory contexts, where acidic microenvironments are common, and it has been studied in relation to endothelial activation, leukocyte recruitment, and tissue responses to hypoxia and inflammation. Altered GPR4 signaling has been associated with dysregulated inflammatory signaling and microenvironmental stress responses relevant to cardiovascular and immune-mediated disease biology.
GPR4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPR4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPR4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPR4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR4 protein expression.
This CRISPR knockout system enables efficient generation of GPR4-deficient cell models for investigation of GPR4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPR4 exon(s) critical for GPR4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPR4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR4 CRISPR/Cas9 KO Plasmid (h) and GPR4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPR4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR4 HDR Plasmid (h) and GPR4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPR4 homology arms to support homology-directed repair at defined GPR4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.