GPR172A CRISPR/Cas9 KO Plasmid (h): sc-405622
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR172A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR172A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR172A CRISPR/Cas9 KO Plasmid (h) | sc-405622 | 20 µg | $397.00 |
Overview
SLC52A2 encodes the human riboflavin transporter protein GPR172A (RFVT2), a plasma membrane carrier that mediates cellular uptake of riboflavin (vitamin B2), a precursor for the essential flavin cofactors FMN and FAD. By sustaining flavoprotein-dependent redox reactions, mitochondrial oxidative metabolism, and antioxidant defense, GPR172A supports pathways including electron transport, fatty acid oxidation, and broader cellular energy homeostasis. Disruption of riboflavin transport perturbs flavin-dependent enzyme activities and is linked to neurometabolic dysfunction. Genetic variation in SLC52A2 is associated with riboflavin transporter deficiency syndromes affecting peripheral nerves and central nervous system function, making it relevant for studies of nutrient transport and mitochondrial stress responses.
GPR172A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC52A2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC52A2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC52A2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR172A protein expression.
This CRISPR knockout system enables efficient generation of SLC52A2-deficient cell models for investigation of GPR172A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC52A2 exon(s) critical for GPR172A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC52A2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR172A CRISPR/Cas9 KO Plasmid (h) and GPR172A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC52A2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR172A HDR Plasmid (h) and GPR172A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC52A2 homology arms to support homology-directed repair at defined SLC52A2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.