GPR158 Double Nickase Plasmid (m): sc-433824-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR158 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR158 Double Nickase Plasmid (m) and GPR158 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Gpr158. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR158 Double Nickase Plasmid (m) | sc-433824-NIC | 20 µg | $410.00 | |||
GPR158 Double Nickase Plasmid (m2) | sc-433824-NIC-2 | 20 µg | $410.00 |
Mouse Gpr158 encodes GPR158, an orphan class C G protein–coupled receptor enriched in the nervous system that functions as a signaling scaffold integrating extracellular cues with intracellular effector pathways. GPR158 has been linked to modulation of cAMP signaling and MAPK/ERK-associated processes, influencing neuronal excitability, synaptic organization, and experience-dependent plasticity. Through interactions with regulatory proteins and synaptic components, it contributes to circuit-level adaptations relevant to stress responsivity and cognitive behaviors. Dysregulation of GPR158-associated signaling has been studied in the context of neuropsychiatric and neurodevelopmental phenotypes, supporting its use in mechanistic neuroscience and functional genomics research.
GPR158 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Gpr158 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Gpr158. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Gpr158 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Gpr158-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.