Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GPD1 CRISPR/Cas9 KO Plasmid (h): sc-403706
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPD1 Antibody (E-7): sc-376219
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPD1 CRISPR/Cas9 KO Plasmid (h) | sc-403706 | 20 µg | $397.00 |
Overview
Human GPD1 encodes cytosolic glycerol-3-phosphate dehydrogenase 1, an NADH-dependent enzyme that interconverts dihydroxyacetone phosphate and glycerol-3-phosphate, linking glycolysis to glycerolipid synthesis and redox balancing. Through its role in the glycerol phosphate shuttle and triglyceride/phospholipid precursor generation, GPD1 influences cytosolic NAD+/NADH homeostasis, energy metabolism, and lipid storage programs. Altered GPD1 activity has been connected to metabolic remodeling in liver and adipose tissues and is studied in the context of dyslipidemia, insulin resistance–associated pathways, and broader mitochondrial–cytosolic redox coupling. These functions make GPD1 a useful node for investigating carbon flux partitioning between glycolysis and lipid biosynthesis under nutrient stress or hormonal signaling.
GPD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPD1 protein expression.
This CRISPR knockout system enables efficient generation of GPD1-deficient cell models for investigation of GPD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPD1 exon(s) critical for GPD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPD1 CRISPR/Cas9 KO Plasmid (h) and GPD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPD1 HDR Plasmid (h) and GPD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPD1 homology arms to support homology-directed repair at defined GPD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.