Glycophorin C Double Nickase Plasmid (h): sc-405123-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glycophorin C Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Glycophorin C Double Nickase Plasmid (h) and Glycophorin C Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GYPC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Glycophorin C/D Antibody (F-8): sc-365599
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glycophorin C Double Nickase Plasmid (h) | sc-405123-NIC | 20 µg | $410.00 | |||
Glycophorin C Double Nickase Plasmid (h2) | sc-405123-NIC-2 | 20 µg | $410.00 |
GYPC encodes glycophorin C, a major sialoglycoprotein of the human erythrocyte membrane that contributes to membrane stability and surface charge through its heavily glycosylated extracellular domain. Glycophorin C associates with the membrane–cytoskeleton network, including interactions linked to protein 4.1R, and supports red blood cell deformability under shear stress. Variation or deficiency in GYPC can alter erythrocyte mechanical properties and antigen presentation, making it relevant to studies of hereditary red cell membrane disorders and blood group biology. As a prominent red cell surface determinant, glycophorin C also serves as a model for investigating glycoprotein trafficking, membrane organization, and host–pathogen interactions at the erythrocyte interface.
Glycophorin C Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GYPC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GYPC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GYPC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GYPC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.