Glucose Transporter Glut2 Double Nickase Plasmid (h): sc-400347-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glucose Transporter Glut2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Glucose Transporter Glut2 Double Nickase Plasmid (h) and Glucose Transporter Glut2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC2A2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Glucose Transporter Glut2 Antibody (C-10): sc-518022
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glucose Transporter Glut2 Double Nickase Plasmid (h) | sc-400347-NIC | 20 µg | $410.00 | |||
Glucose Transporter Glut2 Double Nickase Plasmid (h2) | sc-400347-NIC-2 | 20 µg | $410.00 |
SLC2A2 encodes glucose transporter GLUT2, a facilitative hexose transporter that mediates bidirectional glucose flux across the plasma membrane in metabolically active tissues, supporting glucose sensing and homeostatic control of carbohydrate metabolism. GLUT2 influences cellular energy balance by shaping substrate availability for glycolysis and downstream metabolic signaling, and it contributes to nutrient-responsive transcriptional programs in endocrine and epithelial contexts. Altered SLC2A2 expression or function has been associated with dysregulated glucose handling and phenotypes relevant to diabetes and related metabolic disorders, as well as transporter-dependent effects on epithelial transport physiology. In human cell models, SLC2A2 perturbation is commonly used to dissect glucose uptake kinetics, metabolic flux, and nutrient-sensing pathways.
Glucose Transporter Glut2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC2A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC2A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC2A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC2A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.