GDF-8/11 Antibody (H-9): sc-393335
- GDF-8/11 Antibody (H-9) is a mouse monoclonal IgG2a κ, cited in 12 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 347-376 at the C-terminus of GDF-8 of mouse origin
- Anti-GDF-8/11 Antibody (H-9) is recommended for detection of precursor and mature GDF-8 and GDF-11 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-GDF-8/11 Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP is the preferred secondary detection reagent for GDF-8/11 Antibody (H-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with GDF-8/11 Antibody (H-9) (see ordering information below).
GDF-8/11 Antibody (H-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects GDF-8/11 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GDF-8/11 (H-9) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. The growth/differentiation factors (GDFs), which belong to the transforming growth factor-beta (TGF-β) superfamily, play crucial roles in embryonic development and the maintenance of adult tissue homeostasis. GDF-8, commonly referred to as myostatin, serves as a negative regulator of skeletal muscle growth, making GDF-8 vital for understanding muscle development and potential therapeutic targets for muscle-wasting diseases. GDF-11 is essential for anterior/posterior patterning of the axial skeleton and regulates organogenesis in the kidney and pancreas by influencing the expression of glial cell line-derived neurotrophic factor (GDNF). GDF-11 is involved in the control of HOX gene expression, induces phosphorylation of Smad2, mediates Nodal signaling during left-right patterning, and plays a role in the development of head structures while inhibiting the generation of new neurons from progenitor cells in the olfactory epithelium.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.
Download thiomersal SDS
GDF-8/11 Antibody (H-9) References:
- Characterization of amphioxus GDF8/11 gene, an archetype of vertebrate MSTN and GDF11. | Xing, F., et al. 2007. Dev Genes Evol. 217: 549-54. PMID: 17551751
- Structural basis for potency differences between GDF8 and GDF11. | Walker, RG., et al. 2017. BMC Biol. 15: 19. PMID: 28257634
- Interaction with the GDF8/11 pathway reveals treatment options for adenocarcinoma of the breast. | Wallner, C., et al. 2018. Breast. 37: 134-141. PMID: 29156385
- Crystal structure of the WFIKKN2 follistatin domain reveals insight into how it inhibits growth differentiation factor 8 (GDF8) and GDF11. | McCoy, JC., et al. 2019. J Biol Chem. 294: 6333-6343. PMID: 30814254
- Targeting Unique Epitopes on Highly Similar Proteins GDF-11 and GDF-8 with Modified DNA Aptamers. | Ochsner, UA., et al. 2019. Biochemistry. 58: 4632-4640. PMID: 31638376
- Targeted Approach to Distinguish and Determine Absolute Levels of GDF8 and GDF11 in Mouse Serum. | Camparini, L., et al. 2020. Proteomics. 20: e1900104. PMID: 32104967
- Exogenous GDF11, but not GDF8, reduces body weight and improves glucose homeostasis in mice. | Walker, RG., et al. 2020. Sci Rep. 10: 4561. PMID: 32165710
- ACTRIIA-Fc rebalances activin/GDF versus BMP signaling in pulmonary hypertension. | Yung, LM., et al. 2020. Sci Transl Med. 12: PMID: 32404506
- Functional substitutions of amino acids that differ between GDF11 and GDF8 impact skeletal development and skeletal muscle. | Lian, J., et al. 2023. Life Sci Alliance. 6: PMID: 36631218
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GDF-8/11 Antibody (H-9) | sc-393335 | 200 µg/ml | $322.00 | |||
GDF-8/11 Antibody (H-9): m-IgG2a BP-HRP Bundle | sc-545965 | 200 µg Ab; 10 µg BP | $361.00 | |||
GDF-8/11 Antibody (H-9) AC | sc-393335 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GDF-8/11 Antibody (H-9) HRP | sc-393335 HRP | 200 µg/ml | $322.00 | |||
GDF-8/11 Antibody (H-9) FITC | sc-393335 FITC | 200 µg/ml | $336.00 | |||
GDF-8/11 Antibody (H-9) PE | sc-393335 PE | 200 µg/ml | $349.00 | |||
GDF-8/11 Antibody (H-9) Alexa Fluor® 488 | sc-393335 AF488 | 200 µg/ml | $364.00 | |||
GDF-8/11 Antibody (H-9) Alexa Fluor® 546 | sc-393335 AF546 | 200 µg/ml | $364.00 | |||
GDF-8/11 Antibody (H-9) Alexa Fluor® 594 | sc-393335 AF594 | 200 µg/ml | $364.00 | |||
GDF-8/11 Antibody (H-9) Alexa Fluor® 647 | sc-393335 AF647 | 200 µg/ml | $364.00 | |||
GDF-8/11 Antibody (H-9) Alexa Fluor® 680 | sc-393335 AF680 | 200 µg/ml | $364.00 | |||
GDF-8/11 Antibody (H-9) Alexa Fluor® 790 | sc-393335 AF790 | 200 µg/ml | $364.00 | |||
GDF-8/11 (H-9) Neutralizing Peptide | sc-393335 P | 100 µg/0.5 ml | $69.00 |