GD3 synthase Double Nickase Plasmid (m): sc-422942-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GD3 synthase Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GD3 synthase Double Nickase Plasmid (m) and GD3 synthase Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting St8sia1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GD3 Synthase Antibody (B-11): sc-390123
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GD3 synthase Double Nickase Plasmid (m) | sc-422942-NIC | 20 µg | $410.00 | |||
GD3 synthase Double Nickase Plasmid (m2) | sc-422942-NIC-2 | 20 µg | $410.00 |
Mouse St8sia1 encodes GD3 synthase (ST8SIA1), an α2,8-sialyltransferase that converts GM3 to GD3 and initiates biosynthesis of b- and c-series gangliosides in the Golgi. By shaping ganglioside composition at the plasma membrane, GD3 synthase influences lipid raft organization, receptor signaling, and downstream processes including cell adhesion, migration, and differentiation. St8sia1 activity intersects glycosphingolipid metabolism and immune and neural pathways where gangliosides modulate growth factor and cytokine responses. Altered ganglioside profiles involving GD3 have been associated with dysregulated signaling and cellular stress programs relevant to neurodevelopmental and oncogenic model systems, supporting its use in mechanistic studies of membrane glycosylation.
GD3 synthase Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the St8sia1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within St8sia1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt St8sia1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of St8sia1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.