GCM1 CRISPR/Cas9 KO Plasmid (m): sc-420510
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GCM1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GCM1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCM1 CRISPR/Cas9 KO Plasmid (m) | sc-420510 | 20 µg | $397.00 |
Overview
Gcm1 encodes the transcription factor GCM1, a DNA-binding regulator essential for placental trophoblast differentiation and labyrinth formation in mouse development. GCM1 controls gene programs involved in cell fate specification, syncytiotrophoblast formation, and branching morphogenesis, coordinating expression of pathways that govern cell fusion, nutrient exchange, and vascular interface maturation. Dysregulated GCM1 activity is linked to abnormal placentation and impaired fetal growth, making it relevant to studies of developmental disorders and placental pathophysiology. In addition to its developmental role, GCM1-dependent transcriptional networks provide a tractable system for dissecting lineage-specific gene regulation.
GCM1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gcm1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gcm1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gcm1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GCM1 protein expression.
This CRISPR knockout system enables efficient generation of Gcm1-deficient cell models for investigation of GCM1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gcm1 exon(s) critical for GCM1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gcm1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GCM1 CRISPR/Cas9 KO Plasmid (m) and GCM1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gcm1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GCM1 HDR Plasmid (m) and GCM1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gcm1 homology arms to support homology-directed repair at defined Gcm1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.