GATAD1 CRISPR/Cas9 KO Plasmid (h): sc-403468
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GATAD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GATAD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GATAD1 Antibody (GATA9A1D3): sc-81092
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GATAD1 CRISPR/Cas9 KO Plasmid (h) | sc-403468 | 20 µg | $397.00 |
Overview
GATAD1 (GATA zinc finger domain containing 1) encodes a nuclear chromatin-associated protein implicated in transcriptional regulation through DNA binding and protein–protein interactions. Via its zinc finger domain, GATAD1 is linked to control of gene expression programs that influence cell proliferation, differentiation, and stress-adaptive responses, often in coordination with epigenetic modifiers and chromatin remodeling complexes. Altered GATAD1 expression or copy number has been reported in multiple tumor contexts, supporting interest in its contribution to oncogenic transcriptional states and genome-wide regulatory network perturbations. As a result, GATAD1 is commonly studied in mechanisms of chromatin regulation, transcription factor circuitry, and cancer-relevant signaling cross-talk.
GATAD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GATAD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GATAD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GATAD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GATAD1 protein expression.
This CRISPR knockout system enables efficient generation of GATAD1-deficient cell models for investigation of GATAD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GATAD1 exon(s) critical for GATAD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GATAD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GATAD1 CRISPR/Cas9 KO Plasmid (h) and GATAD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GATAD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GATAD1 HDR Plasmid (h) and GATAD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GATAD1 homology arms to support homology-directed repair at defined GATAD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.