GART Double Nickase Plasmid (h): sc-403800-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GART Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GART Double Nickase Plasmid (h) and GART Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GART. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GART Antibody (D-4): sc-166379
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GART Double Nickase Plasmid (h) | sc-403800-NIC | 20 µg | $410.00 | |||
GART Double Nickase Plasmid (h2) | sc-403800-NIC-2 | 20 µg | $410.00 |
Human GART encodes a trifunctional enzyme that catalyzes key steps in the de novo purine biosynthesis pathway, supporting production of inosine monophosphate and downstream adenine and guanine nucleotides. By controlling nucleotide availability, GART influences DNA and RNA synthesis, ATP/GTP-dependent signaling, and one-carbon metabolism through folate-linked reactions. GART activity is tightly coupled to proliferative demands and broader metabolic programs that coordinate biosynthetic flux with cell-cycle progression. Altered purine metabolism and gene dosage at the GART locus have been linked to metabolic dysregulation and neurodevelopmental phenotypes, making GART a relevant target for mechanistic studies of nucleotide homeostasis.
GART Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GART locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GART. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GART function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GART-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.