FUCA2 CRISPR/Cas9 KO Plasmid (h): sc-405221
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FUCA2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FUCA2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FUCA2 Antibody (B-11): sc-514038
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FUCA2 CRISPR/Cas9 KO Plasmid (h) | sc-405221 | 20 µg | $397.00 |
Overview
FUCA2 encodes alpha-L-fucosidase 2, a secreted lysosomal-type glycosidase that hydrolyzes terminal fucose residues from N- and O-linked glycoconjugates and glycolipids, contributing to glycan catabolism and remodeling. By modulating fucosylation status on cell-surface and extracellular matrix proteins, FUCA2 can influence processes such as receptor turnover, cell–cell adhesion, and immune-associated glycan signaling. Altered fucose metabolism and fucosidase activity have been linked to changes in inflammatory responses and tumor-associated glycosylation patterns, making FUCA2 a useful node for interrogating glycomics-driven phenotypes. Functional studies commonly focus on how FUCA2-dependent defucosylation impacts trafficking, lysosomal function, and glycoprotein-mediated signaling pathways.
FUCA2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FUCA2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FUCA2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FUCA2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FUCA2 protein expression.
This CRISPR knockout system enables efficient generation of FUCA2-deficient cell models for investigation of FUCA2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FUCA2 exon(s) critical for FUCA2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FUCA2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FUCA2 CRISPR/Cas9 KO Plasmid (h) and FUCA2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FUCA2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FUCA2 HDR Plasmid (h) and FUCA2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FUCA2 homology arms to support homology-directed repair at defined FUCA2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.