FRY CRISPR/Cas9 KO Plasmid (h): sc-404629
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FRY CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FRY genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FRY CRISPR/Cas9 KO Plasmid (h) | sc-404629 | 20 µg | $397.00 |
Overview
FRY (furry homolog) encodes a large, evolutionarily conserved scaffold protein that functions downstream of NDR/LATS family serine/threonine kinases, coordinating signals that regulate cell polarity, cytoskeletal organization, and polarized vesicle trafficking. Through these pathways, FRY contributes to control of cell morphogenesis, migration, and epithelial organization, processes tightly linked to tissue development and homeostasis. Altered FRY function has been associated with dysregulated growth signaling and polarity defects that are frequently implicated in cancer biology and other disorders involving impaired cell architecture. As a modulator of kinase-centered signaling networks, FRY is of interest for mechanistic studies of Hippo-related regulation, cytoskeletal dynamics, and context-specific stress responses.
FRY CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FRY gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FRY together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FRY open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FRY protein expression.
This CRISPR knockout system enables efficient generation of FRY-deficient cell models for investigation of FRY signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FRY exon(s) critical for FRY function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FRY genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FRY CRISPR/Cas9 KO Plasmid (h) and FRY CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FRY locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FRY HDR Plasmid (h) and FRY HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FRY homology arms to support homology-directed repair at defined FRY target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.