FIG4 CRISPR/Cas9 KO Plasmid (h): sc-404404
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FIG4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FIG4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FIG4 CRISPR/Cas9 KO Plasmid (h) | sc-404404 | 20 µg | $397.00 |
Overview
FIG4 encodes a phosphoinositide 5-phosphatase that regulates turnover of phosphatidylinositol 3,5-bisphosphate and related lipids on endolysosomal membranes. Through its participation in PIKfyve–VAC14–FIG4 regulatory complexes, FIG4 influences endosome-to-lysosome trafficking, autophagy, membrane recycling, and maintenance of lysosomal homeostasis. Perturbation of FIG4 activity disrupts endolysosomal dynamics and cellular stress responses, processes central to neuronal maintenance and myelin integrity. Human genetic studies link FIG4 dysfunction to neurodegenerative and neurodevelopmental phenotypes, supporting its importance in membrane trafficking pathways relevant to nervous system biology.
FIG4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FIG4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FIG4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FIG4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FIG4 protein expression.
This CRISPR knockout system enables efficient generation of FIG4-deficient cell models for investigation of FIG4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FIG4 exon(s) critical for FIG4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FIG4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FIG4 CRISPR/Cas9 KO Plasmid (h) and FIG4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FIG4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FIG4 HDR Plasmid (h) and FIG4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FIG4 homology arms to support homology-directed repair at defined FIG4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.