FAM86A CRISPR/Cas9 KO Plasmid (m): sc-427731
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM86A CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAM86A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM86A CRISPR/Cas9 KO Plasmid (m) | sc-427731 | 20 µg | $397.00 |
Overview
Eef2kmt encodes FAM86A, a protein methyltransferase reported to modify translation factor eEF2, linking it to post-translational control of ribosome function and translational elongation. Through regulating eEF2-associated protein homeostasis, FAM86A is positioned to influence cellular growth programs, stress responses, and adaptation to changes in nutrient and energy status. Perturbation of translation control networks is broadly relevant to proliferative and neurobiological phenotypes, making Eef2kmt a useful target for mechanistic studies of proteostasis and signaling crosstalk in mouse systems.
FAM86A CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Eef2kmt gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Eef2kmt together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Eef2kmt open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAM86A protein expression.
This CRISPR knockout system enables efficient generation of Eef2kmt-deficient cell models for investigation of FAM86A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Eef2kmt exon(s) critical for FAM86A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Eef2kmt genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAM86A CRISPR/Cas9 KO Plasmid (m) and FAM86A CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Eef2kmt locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAM86A HDR Plasmid (m) and FAM86A HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Eef2kmt homology arms to support homology-directed repair at defined Eef2kmt target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.