FAM21C CRISPR/Cas9 KO Plasmid (h): sc-404093
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM21C CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAM21C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM21C CRISPR/Cas9 KO Plasmid (h) | sc-404093 | 20 µg | $397.00 |
Overview
WASHC2C encodes FAM21C, a component of the WASH complex that regulates Arp2/3-dependent actin polymerization on endosomes to support retromer-mediated cargo sorting and endosomal tubulation. Through interactions with sorting nexins and retromer subunits, FAM21C contributes to recycling of membrane proteins and maintenance of endolysosomal homeostasis. Disruption of WASH pathway function can perturb receptor trafficking, nutrient transporter recycling, and signaling outputs coupled to endosomal dynamics. Altered endosomal trafficking and cytoskeletal regulation are implicated in cancer biology and neurodegenerative mechanisms, making FAM21C relevant for studies of cellular organization and stress responses.
FAM21C CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the WASHC2C gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the WASHC2C together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the WASHC2C open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAM21C protein expression.
This CRISPR knockout system enables efficient generation of WASHC2C-deficient cell models for investigation of FAM21C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting WASHC2C exon(s) critical for FAM21C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple WASHC2C genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAM21C CRISPR/Cas9 KO Plasmid (h) and FAM21C CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the WASHC2C locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAM21C HDR Plasmid (h) and FAM21C HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by WASHC2C homology arms to support homology-directed repair at defined WASHC2C target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.