EYA4 Double Nickase Plasmid (h): sc-404355-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EYA4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EYA4 Double Nickase Plasmid (h) and EYA4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EYA4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EYA4 Antibody (E-11): sc-393111
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EYA4 Double Nickase Plasmid (h) | sc-404355-NIC | 20 µg | $410.00 | |||
EYA4 Double Nickase Plasmid (h2) | sc-404355-NIC-2 | 20 µg | $410.00 |
EYA4 encodes a member of the Eyes Absent (EYA) family of transcriptional coactivators and haloacid dehalogenase–like phosphatases that function in nucleus–cytoplasm signaling. EYA4 participates in developmental gene control programs by coupling protein tyrosine phosphatase activity with interactions that modulate transcription factor activity and chromatin-associated complexes. In human biology, EYA4 has been linked to sensory and cardiac phenotypes, with genetic variation associated with autosomal dominant hearing loss and reported connections to cardiomyopathy-related processes. Its roles in cell fate specification and stress-responsive transcription make EYA4 a useful target for dissecting regulatory networks in differentiation and disease-relevant cell models.
EYA4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EYA4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EYA4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EYA4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EYA4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.