Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ERRβ Double Nickase Plasmid (h): sc-402868-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ERRβ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ERRβ Double Nickase Plasmid (h) and ERRβ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ESRRB. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERRβ Double Nickase Plasmid (h) | sc-402868-NIC | 20 µg | $410.00 | |||
ERRβ Double Nickase Plasmid (h2) | sc-402868-NIC-2 | 20 µg | $410.00 |
ESRRB encodes estrogen-related receptor beta (ERRβ), an orphan nuclear receptor that functions as a sequence-specific transcription factor regulating gene programs involved in cellular identity, differentiation, and metabolic adaptation. ERRβ participates in nuclear receptor signaling through binding to estrogen-related response elements and modulating transcription in concert with co-regulators, integrating cues from developmental and stress-responsive pathways. In human cells, ESRRB activity has been linked to regulation of stem-like transcriptional networks, lineage commitment, and mitochondrial and oxidative metabolism programs. Dysregulated ESRRB/ERRβ signaling has been explored in contexts of altered differentiation states and proliferative phenotypes, making it relevant for mechanistic studies of transcriptional control in disease-relevant cell models.
ERRβ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ESRRB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ESRRB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ESRRB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ESRRB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.