Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ERAP1 Double Nickase Plasmid (h): sc-403804-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ERAP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ERAP1 Double Nickase Plasmid (h) and ERAP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ERAP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ERAP1 Antibody (B-10): sc-271823
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERAP1 Double Nickase Plasmid (h) | sc-403804-NIC | 20 µg | $410.00 | |||
ERAP1 Double Nickase Plasmid (h2) | sc-403804-NIC-2 | 20 µg | $410.00 |
Endoplasmic reticulum aminopeptidase 1 (ERAP1) is a zinc-dependent M1 aminopeptidase that trims antigenic peptide precursors to optimal lengths for loading onto MHC class I molecules, shaping the cellular immunopeptidome and influencing CD8+ T cell recognition. It functions at the interface of ER protein processing and antigen presentation, acting downstream of proteasomal degradation and TAP-mediated peptide transport as part of the MHC I pathway. Variation in ERAP1 activity can alter peptide repertoire selection and immune signaling thresholds, linking it to immunogenetic susceptibility in several inflammatory and autoimmune contexts, particularly in concert with specific HLA class I alleles. ERAP1 is also studied for roles in ER stress-adjacent processes and innate immune modulation where peptide trimming impacts immune surveillance.
ERAP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ERAP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ERAP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ERAP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ERAP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.