ephrin-B2 Antibody (F-2): sc-398735
- ephrin-B2 Antibody (F-2) is a mouse monoclonal IgG2a κ ephrin-B2 antibody, cited in 13 publications, provided at 200 µg/ml
- raised against amino acids 168-235 of ephrin-B2 of human origin
- ephrin-B2 Antibody (F-2) is recommended for detection of ephrin-B2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ephrin-B2 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for ephrin-B2 Antibody (F-2) for WB applications. These reagents are now offered in bundles with ephrin-B2 Antibody (F-2) (see ordering information below).
ephrin-B2 Antibody (F-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects ephrin-B2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ephrin-B2 Antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ephrins, which serve as ligands for Eph receptors, are critical cell-surface proteins categorized into ephrin-A and ephrin-B based on structural and functional characteristics. ephrin-B2 is particularly significant due to a role in vascular development; ephrin-B2 is a transmembrane protein containing conserved cytoplasmic tyrosine residues that undergo phosphorylation upon binding to EphB receptors. This phosphorylation initiates bidirectional signaling pathways influencing cell positioning and tissue organization during embryogenesis. ephrin-B2 is expressed on arterial endothelial cells, while EphB4 receptor is found on venous endothelial cells, facilitating communication necessary for proper vascular morphogenesis. ephrin-B2 and EphB4 interaction is essential not only for normal embryonic vascular development but also plays a pivotal role in tumor angiogenesis, making ephrin-B2 a significant target in cancer therapeutics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.
Download thiomersal SDS
ephrin-B2 Antibody (F-2) References:
- Eph receptors and ephrins restrict cell intermingling and communication. | Mellitzer, G., et al. 1999. Nature. 400: 77-81. PMID: 10403252
- Eph receptors and ephrins. | Jensen, PL. 2000. Stem Cells. 18: 63-4. PMID: 10661574
- Diverse roles for the Eph family of receptor tyrosine kinases in carcinogenesis. | Nakamoto, M. and Bergemann, AD. 2002. Microsc Res Tech. 59: 58-67. PMID: 12242697
- Distinct roles of ephrin-B2 forward and EphB4 reverse signaling in endothelial cells. | Hamada, K., et al. 2003. Arterioscler Thromb Vasc Biol. 23: 190-7. PMID: 12588758
- Effects of overexpression of ephrin-B2 on tumour growth in human colorectal cancer. | Liu, W., et al. 2004. Br J Cancer. 90: 1620-6. PMID: 15083195
- Bidirectional signaling mediated by ephrin-B2 and EphB2 controls urorectal development. | Dravis, C., et al. 2004. Dev Biol. 271: 272-90. PMID: 15223334
- Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis. | Wang, Y., et al. 2010. Nature. 465: 483-6. PMID: 20445537
- Ephrin-B2-expressing natural killer cells induce angiogenesis. | Wolf, KG., et al. 2022. JVS Vasc Sci. 3: 336-344. PMID: 36439700
- Divergent Roles of Ephrin-B2/EphB4 Guidance System in Pulmonary Hypertension. | Crnkovic, S., et al. 2023. Hypertension. 80: e17-e28. PMID: 36519465
- Sequence basis for selectivity of ephrin-B2 ligand for Eph receptors and pathogenic henipavirus G glycoproteins. | Narayanan, KK., et al. 2023. J Virol. 97: e0062123. PMID: 37931130
- Inhibition of Ephrin B2 Reverse Signaling Abolishes Multiple Myeloma Pathogenesis. | Sasine, JP., et al. 2024. Cancer Res. 84: 919-934. PMID: 38231476
- ephrin-B2 promotes nociceptive plasticity and hyperalgesic priming through EphB2-MNK-eIF4E signaling in both mice and humans. | David, ET., et al. 2024. Pharmacol Res. 206: 107284. PMID: 38925462
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ephrin-B2 Antibody (F-2) | sc-398735 | 200 µg/ml | $322.00 | |||
ephrin-B2 Antibody (F-2): m-IgG Fc BP-HRP Bundle | sc-526322 | 200 µg Ab; 10 µg BP | $361.00 | |||
ephrin-B2 Antibody (F-2): m-IgG2a BP-HRP Bundle | sc-546583 | 200 µg Ab; 10 µg BP | $361.00 | |||
ephrin-B2 Antibody (F-2) AC | sc-398735 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ephrin-B2 Antibody (F-2) HRP | sc-398735 HRP | 200 µg/ml | $322.00 | |||
ephrin-B2 Antibody (F-2) FITC | sc-398735 FITC | 200 µg/ml | $336.00 | |||
ephrin-B2 Antibody (F-2) PE | sc-398735 PE | 200 µg/ml | $349.00 | |||
ephrin-B2 Antibody (F-2) Alexa Fluor® 488 | sc-398735 AF488 | 200 µg/ml | $364.00 | |||
ephrin-B2 Antibody (F-2) Alexa Fluor® 546 | sc-398735 AF546 | 200 µg/ml | $364.00 | |||
ephrin-B2 Antibody (F-2) Alexa Fluor® 594 | sc-398735 AF594 | 200 µg/ml | $364.00 | |||
ephrin-B2 Antibody (F-2) Alexa Fluor® 647 | sc-398735 AF647 | 200 µg/ml | $364.00 | |||
ephrin-B2 Antibody (F-2) Alexa Fluor® 680 | sc-398735 AF680 | 200 µg/ml | $364.00 | |||
ephrin-B2 Antibody (F-2) Alexa Fluor® 790 | sc-398735 AF790 | 200 µg/ml | $364.00 |