Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EphB3 Double Nickase Plasmid (h): sc-403039-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EphB3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EphB3 Double Nickase Plasmid (h) and EphB3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EPHB3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EphB3 Antibody (A-12): sc-514139
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EphB3 Double Nickase Plasmid (h) | sc-403039-NIC | 20 µg | $410.00 | |||
EphB3 Double Nickase Plasmid (h2) | sc-403039-NIC-2 | 20 µg | $410.00 |
EPHB3 encodes the EphB3 receptor tyrosine kinase, a member of the ephrin receptor family that mediates contact-dependent signaling upon binding membrane-tethered ephrin-B ligands. EphB3 regulates cell–cell communication, cytoskeletal remodeling, adhesion, and directional migration through pathways that intersect with Rho family GTPases, MAPK/ERK signaling, and PI3K-associated networks. In development and tissue homeostasis, EphB3 contributes to boundary formation, axon guidance, and epithelial organization, and its dysregulation is linked to altered invasion and differentiation programs in multiple cancer contexts. These properties make EPHB3 a useful target for dissecting receptor-driven signaling outputs and cell behavior in model systems.
EphB3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EPHB3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EPHB3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EPHB3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EPHB3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.