Eos CRISPR/Cas9 KO Plasmid (h): sc-402330
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Eos CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Eos genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Eos CRISPR/Cas9 KO Plasmid (h) | sc-402330 | 20 µg | $397.00 |
Overview
IKZF4 (Eos) is a zinc finger transcription factor of the Ikaros family that regulates gene expression programs controlling lymphocyte differentiation, activation, and tolerance. In human T cells, Eos is prominently expressed in regulatory T cells and contributes to maintaining suppressive identity by coordinating transcriptional repression and chromatin-associated control of immune effector genes. Through integration with transcriptional and epigenetic networks, IKZF4 influences cytokine signaling responsiveness, TCR-driven activation states, and lineage stability. Dysregulated IKZF4/Eos function has been associated with altered immune homeostasis and has been studied in contexts of autoimmunity, inflammation, and immune-oncology biology.
Eos CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IKZF4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IKZF4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IKZF4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Eos protein expression.
This CRISPR knockout system enables efficient generation of IKZF4-deficient cell models for investigation of Eos signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IKZF4 exon(s) critical for Eos function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IKZF4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Eos CRISPR/Cas9 KO Plasmid (h) and Eos CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IKZF4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Eos HDR Plasmid (h) and Eos HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IKZF4 homology arms to support homology-directed repair at defined IKZF4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.