ENC1 CRISPR/Cas9 KO Plasmid (m): sc-420175
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ENC1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ENC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ENC1 Antibody (7a): sc-517590
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ENC1 CRISPR/Cas9 KO Plasmid (m) | sc-420175 | 20 µg | $397.00 |
Overview
ENC1 (ectodermal-neural cortex 1) encodes a kelch repeat–containing protein that functions as an actin-associated adaptor involved in cytoskeletal organization and cellular differentiation programs. In mouse tissues, ENC1 expression is enriched in neural and other differentiating lineages, where it has been linked to modulation of oxidative stress responses and protein homeostasis. Reported interactions and pathway associations place ENC1 in processes governing cell shape changes, neurite outgrowth, and regulated turnover of cytoskeleton-associated proteins, including crosstalk with ubiquitin-dependent mechanisms. Dysregulated ENC1 expression has been observed in contexts of neurodevelopmental alterations and tumor biology, supporting its use as a molecular handle to study differentiation state and stress-adaptive signaling.
ENC1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Enc1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Enc1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Enc1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ENC1 protein expression.
This CRISPR knockout system enables efficient generation of Enc1-deficient cell models for investigation of ENC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Enc1 exon(s) critical for ENC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Enc1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ENC1 CRISPR/Cas9 KO Plasmid (m) and ENC1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Enc1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ENC1 HDR Plasmid (m) and ENC1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Enc1 homology arms to support homology-directed repair at defined Enc1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.