Enamelin CRISPR/Cas9 KO Plasmid (m): sc-420174
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Enamelin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Enamelin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Enamelin CRISPR/Cas9 KO Plasmid (m) | sc-420174 | 20 µg | $397.00 |
Overview
Enam encodes enamelin, a secreted extracellular matrix glycoprotein essential for dental enamel formation in mouse. Enamelin participates in enamel biomineralization by regulating hydroxyapatite crystal nucleation and growth and coordinating the assembly and processing of the enamel matrix in concert with other amelogenesis proteins. Its activity supports ameloblast-driven matrix deposition, proteolytic remodeling, and mineral maturation processes that define enamel thickness and structural integrity. Disruption of ENAM function is linked to enamel hypoplasia and amelogenesis imperfecta-like phenotypes, making it a useful target for studying mineralized tissue development and tooth pathology mechanisms.
Enamelin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Enam gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Enam together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Enam open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Enamelin protein expression.
This CRISPR knockout system enables efficient generation of Enam-deficient cell models for investigation of Enamelin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Enam exon(s) critical for Enamelin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Enam genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Enamelin CRISPR/Cas9 KO Plasmid (m) and Enamelin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Enam locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Enamelin HDR Plasmid (m) and Enamelin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Enam homology arms to support homology-directed repair at defined Enam target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.