Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Emx1 Double Nickase Plasmid (h): sc-402711-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Emx1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Emx1 Double Nickase Plasmid (h) and Emx1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EMX1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Emx1 Antibody (G-6): sc-398115
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Emx1 Double Nickase Plasmid (h) | sc-402711-NIC | 20 µg | $410.00 | |||
Emx1 Double Nickase Plasmid (h2) | sc-402711-NIC-2 | 20 µg | $410.00 |
EMX1 encodes the homeobox transcription factor Emx1, a sequence-specific DNA-binding protein that helps govern dorsal telencephalon patterning and corticogenesis during human neurodevelopment. Emx1 regulates gene expression programs linked to neuronal fate specification, progenitor proliferation, and regional identity, integrating with broader transcriptional networks that coordinate forebrain development. Perturbation of EMX1-associated regulatory circuits is relevant to studying mechanisms underlying neurodevelopmental phenotypes and altered cortical organization, and EMX1 is frequently used as a benchmark locus for evaluating genome engineering performance in human cells. As a nuclear transcription factor, Emx1 provides a tractable model for interrogating how developmental transcriptional programs shape cell state transitions and lineage decisions.
Emx1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EMX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EMX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EMX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EMX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.