Elongin A3 CRISPR/Cas9 KO Plasmid (h): sc-418260
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Elongin A3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Elongin A3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Elongin A3 CRISPR/Cas9 KO Plasmid (h) | sc-418260 | 20 µg | $397.00 |
Overview
TCEB3C encodes human Elongin A3, a member of the Elongin transcription elongation factor complex that supports RNA polymerase II processivity and coordinates transcription with co‑transcriptional quality control. Elongin family proteins interface with ubiquitin-dependent regulatory mechanisms and help shape cellular programs linked to stress responses, proteostasis, and cell-cycle control. As a transcription-associated factor, Elongin A3 is relevant to studies of gene expression regulation and chromatin-linked signaling pathways that influence proliferation and differentiation. Dysregulation of transcription elongation and ubiquitin-mediated control is frequently implicated in oncogenic and neurodegenerative biology, making TCEB3C a useful locus for mechanistic investigation in disease-relevant models.
Elongin A3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TCEB3C gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TCEB3C together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TCEB3C open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Elongin A3 protein expression.
This CRISPR knockout system enables efficient generation of TCEB3C-deficient cell models for investigation of Elongin A3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TCEB3C exon(s) critical for Elongin A3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TCEB3C genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Elongin A3 CRISPR/Cas9 KO Plasmid (h) and Elongin A3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TCEB3C locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Elongin A3 HDR Plasmid (h) and Elongin A3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TCEB3C homology arms to support homology-directed repair at defined TCEB3C target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.