DYNC2H1 CRISPR/Cas9 KO Plasmid (h): sc-406180
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DYNC2H1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DYNC2H1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DYNC2H1 CRISPR/Cas9 KO Plasmid (h) | sc-406180 | 20 µg | $397.00 |
Overview
DYNC2H1 encodes the cytoplasmic dynein 2 heavy chain, the primary retrograde motor of the intraflagellar transport (IFT) machinery that powers trafficking from the ciliary tip to the base. By coupling ATP-driven motility to IFT-A and cargo adaptor complexes, DYNC2H1 is essential for assembly and maintenance of primary cilia and for cilia-dependent signal transduction, including Hedgehog pathway dynamics. Disruption of DYNC2H1 compromises ciliary structure and alters developmental signaling outputs, linking the gene to human ciliopathies characterized by skeletal and developmental phenotypes. These functions make DYNC2H1 a useful node for studying cilia biology, organelle transport, and pathway-regulated transcriptional programs.
DYNC2H1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DYNC2H1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DYNC2H1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DYNC2H1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DYNC2H1 protein expression.
This CRISPR knockout system enables efficient generation of DYNC2H1-deficient cell models for investigation of DYNC2H1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DYNC2H1 exon(s) critical for DYNC2H1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DYNC2H1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DYNC2H1 CRISPR/Cas9 KO Plasmid (h) and DYNC2H1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DYNC2H1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DYNC2H1 HDR Plasmid (h) and DYNC2H1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DYNC2H1 homology arms to support homology-directed repair at defined DYNC2H1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.