Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dynactin p62 Double Nickase Plasmid (m): sc-426674-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dynactin p62 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dynactin p62 Double Nickase Plasmid (m) and Dynactin p62 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Dctn4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dynactin p62 Antibody (H-4): sc-55603
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynactin p62 Double Nickase Plasmid (m) | sc-426674-NIC | 20 µg | $410.00 | |||
Dynactin p62 Double Nickase Plasmid (m2) | sc-426674-NIC-2 | 20 µg | $410.00 |
Mouse Dctn4 encodes dynactin p62, a dynactin complex subunit that supports cytoplasmic dynein-driven transport along microtubules. Dynactin p62 contributes to cargo binding and processive motility of dynein–dynactin assemblies, influencing endosome and lysosome positioning, vesicle trafficking, and autophagy-associated cargo handling. Through these functions, Dctn4 impacts neuronal and immune cell homeostasis where long-range transport and organelle distribution are critical. Disruption of dynein–dynactin pathway components is broadly linked to neurodegenerative phenotypes, altered proteostasis, and inflammatory signaling, making Dctn4 a useful node for mechanistic studies of microtubule-based transport defects.
Dynactin p62 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Dctn4 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Dctn4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Dctn4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Dctn4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.