DTWD1 Double Nickase Plasmid (h): sc-416659-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DTWD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DTWD1 Double Nickase Plasmid (h) and DTWD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DTWD1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DTWD1 Antibody (E-5): sc-398408
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DTWD1 Double Nickase Plasmid (h) | sc-416659-NIC | 20 µg | $410.00 | |||
DTWD1 Double Nickase Plasmid (h2) | sc-416659-NIC-2 | 20 µg | $410.00 |
DTWD1 encodes a conserved DTW domain–containing protein implicated in RNA metabolism, with proposed roles in tRNA and broader RNA modification processes that can influence translational fidelity and proteostasis. Although DTWD1 is not fully characterized, DTW domain proteins are commonly linked to nucleotide-modifying activities and coordination of RNA-processing pathways within the cytoplasm and nucleus. Perturbation of RNA modification and translation control can reshape cell-cycle progression and stress-response signaling, making DTWD1 relevant to studies of gene expression regulation and cellular homeostasis. Altered DTWD1 expression has been reported in transcriptomic datasets across multiple disease contexts, supporting its use as a candidate gene in mechanistic genomics investigations.
DTWD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DTWD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DTWD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DTWD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DTWD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.