Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
dsg1 Double Nickase Plasmid (h): sc-401442-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- dsg1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- dsg1 Double Nickase Plasmid (h) and dsg1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DSG1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: dsg1 Antibody (B-11): sc-137164
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
dsg1 Double Nickase Plasmid (h) | sc-401442-NIC | 20 µg | $410.00 | |||
dsg1 Double Nickase Plasmid (h2) | sc-401442-NIC-2 | 20 µg | $410.00 |
Desmoglein-1 (DSG1; dsg1) is a calcium-dependent desmosomal cadherin that mediates strong cell–cell adhesion in stratified epithelia, helping organize desmosome assembly and maintain epidermal barrier integrity. By coupling to plakoglobin, plakophilins, and desmoplakin, DSG1 links intercellular junctions to the keratin intermediate filament network and supports tissue resilience under mechanical stress. DSG1 function intersects with epithelial differentiation programs and junctional remodeling processes that influence signaling through pathways such as EGFR/MAPK and responses to inflammatory cues. Altered DSG1 expression or function is associated with skin barrier defects and desmosome-related blistering and keratoderma phenotypes, making it a relevant target for mechanistic studies of adhesion and epidermal homeostasis.
dsg1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DSG1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DSG1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DSG1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DSG1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.