DP2 Double Nickase Plasmid (h): sc-404990-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DP2 Double Nickase Plasmid (h) and DP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTGDR2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DP2 Antibody (C-5): sc-271898
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DP2 Double Nickase Plasmid (h) | sc-404990-NIC | 20 µg | $410.00 | |||
DP2 Double Nickase Plasmid (h2) | sc-404990-NIC-2 | 20 µg | $410.00 |
PTGDR2 encodes DP2 (also known as CRTH2), a G protein–coupled receptor that binds prostaglandin D2 and related lipid mediators to regulate chemotaxis, activation, and cytokine programs in type 2 immune cells, including Th2 lymphocytes, eosinophils, and basophils. DP2 signaling engages cAMP-dependent pathways and downstream effectors such as MAPK and PI3K modules, shaping inflammatory cell recruitment and effector function at mucosal surfaces. This axis is frequently studied in the context of allergic inflammation and broader immune dysregulation, where altered prostaglandin signaling can influence leukocyte trafficking and tissue remodeling. As a membrane receptor with defined ligand-driven signaling, DP2 provides a tractable node for dissecting eicosanoid-mediated communication between stromal and immune compartments.
DP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTGDR2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTGDR2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTGDR2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTGDR2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.