DOC1 Double Nickase Plasmid (h): sc-407358-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DOC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DOC1 Double Nickase Plasmid (h) and DOC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FILIP1L. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DOC1 Antibody (D-2): sc-376472
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DOC1 Double Nickase Plasmid (h) | sc-407358-NIC | 20 µg | $410.00 |
FILIP1L (DOC1) encodes filamin A interacting protein 1-like, a cytoskeletal-associated regulator implicated in actin remodeling, cell shape control, and adhesion-dependent signaling. DOC1 has been linked to modulation of cell migration and proliferation through pathways that coordinate extracellular matrix interactions and Rho family GTPase-driven dynamics. Altered FILIP1L expression has been reported in multiple cancer contexts and is studied in relation to invasion, metastasis-associated phenotypes, and stress-responsive transcriptional programs. These features make DOC1 a useful node for investigating how cytoskeletal organization integrates with growth and motility control in human cells.
DOC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FILIP1L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FILIP1L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FILIP1L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FILIP1L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.