DnaJB3 CRISPR/Cas9 KO Plasmid (m): sc-420974
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DnaJB3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DnaJB3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DnaJB3 CRISPR/Cas9 KO Plasmid (m) | sc-420974 | 20 µg | $397.00 |
Overview
Dnajb3 encodes DnaJB3, a member of the Hsp40/DnaJ co-chaperone family that partners with Hsp70 to regulate protein folding, refolding, and triage of misfolded polypeptides. Through its J-domain–mediated stimulation of Hsp70 ATPase activity, DnaJB3 contributes to cellular proteostasis and supports stress-responsive pathways linked to protein quality control. These processes intersect with endoplasmic reticulum stress responses and proteolytic clearance mechanisms that influence cell survival under proteotoxic conditions. Altered chaperone network function is broadly relevant to models of metabolic dysfunction and neurodegeneration where impaired proteostasis and stress signaling contribute to disease-associated phenotypes.
DnaJB3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dnajb3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dnajb3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dnajb3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DnaJB3 protein expression.
This CRISPR knockout system enables efficient generation of Dnajb3-deficient cell models for investigation of DnaJB3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dnajb3 exon(s) critical for DnaJB3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dnajb3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DnaJB3 CRISPR/Cas9 KO Plasmid (m) and DnaJB3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dnajb3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DnaJB3 HDR Plasmid (m) and DnaJB3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dnajb3 homology arms to support homology-directed repair at defined Dnajb3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.