Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DHS Double Nickase Plasmid (h): sc-402858-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DHS Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DHS Double Nickase Plasmid (h) and DHS Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DHPS. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DHS Antibody (A-10): sc-365077
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DHS Double Nickase Plasmid (h) | sc-402858-NIC | 20 µg | $410.00 | |||
DHS Double Nickase Plasmid (h2) | sc-402858-NIC-2 | 20 µg | $410.00 |
Human DHPS encodes deoxyhypusine synthase (DHS), an essential NAD-dependent enzyme that catalyzes the first step of hypusination by transferring an aminobutyl moiety from spermidine to eIF5A, generating deoxyhypusine. This post-translational modification is required for eIF5A activity and supports translation elongation, protein synthesis control, and cell-cycle progression, linking polyamine metabolism to proteostasis. DHS function intersects with pathways governing cellular growth and stress responses, and perturbation of the DHPS–eIF5A hypusination axis has been associated with dysregulated proliferation and neurodevelopmental phenotypes. Because hypusination impacts selective translation of difficult-to-synthesize motifs, DHPS is frequently studied in the context of metabolic rewiring, proteotoxic stress, and mechanisms that maintain tissue homeostasis.
DHS Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DHPS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DHPS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DHPS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DHPS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.