Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DDX3X Double Nickase Plasmid (h): sc-401253-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX3X Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDX3X Double Nickase Plasmid (h) and DDX3X Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DDX3X. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDX3 Antibody (2253C5a): sc-81247
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX3X Double Nickase Plasmid (h) | sc-401253-NIC | 20 µg | $410.00 | |||
DDX3X Double Nickase Plasmid (h2) | sc-401253-NIC-2 | 20 µg | $410.00 |
DDX3X encodes an ATP-dependent DEAD-box RNA helicase that coordinates multiple steps of RNA metabolism, including pre-mRNA splicing, mRNA export, translation initiation, and stress granule dynamics. Through remodeling RNA–protein complexes, DDX3X integrates signaling cues that affect cell-cycle progression, innate immune sensing, and cellular stress responses. Dysregulation or mutation of DDX3X has been linked to neurodevelopmental phenotypes and has been recurrently observed across diverse cancer contexts, reflecting its broad influence on RNA processing and proteostasis. These properties make DDX3X a useful node for studying RNA helicase function, translational control, and pathways that couple RNA regulation to proliferation and stress adaptation.
DDX3X Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DDX3X locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DDX3X. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DDX3X function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DDX3X-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.