DDR1 Double Nickase Plasmid (h): sc-400517-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDR1 Double Nickase Plasmid (h) and DDR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DDR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDR1 Antibody (C-6): sc-374618
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDR1 Double Nickase Plasmid (h) | sc-400517-NIC | 20 µg | $410.00 | |||
DDR1 Double Nickase Plasmid (h2) | sc-400517-NIC-2 | 20 µg | $410.00 |
DDR1 (discoidin domain receptor 1) is a collagen-activated receptor tyrosine kinase that functions as an extracellular matrix sensor linking collagen engagement to intracellular signaling. Upon activation, DDR1 undergoes autophosphorylation and propagates pathways involved in cell adhesion, cytoskeletal remodeling, migration, and survival, with documented crosstalk to MAPK/ERK, PI3K–AKT, and focal adhesion signaling. DDR1 activity contributes to regulation of epithelial and stromal interactions, basement membrane organization, and matrix remodeling in normal tissue homeostasis. Altered DDR1 expression or signaling has been associated with fibrosis and tumor-associated invasion and metastasis, making it a relevant node for studying microenvironment-driven phenotypes.
DDR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DDR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DDR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DDR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DDR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.