Anticorpo DAT/Dopamine Transporter/SLC6A3 (6-8D6) raccomandato per il rilevamento di DAT di origine mouse, rat e human in WB, IP, IF e IHC(P)

Anticorpo DAT/Dopamine Transporter/SLC6A3 (6-8D6): sc-32259

Anticorpo DAT/Dopamine Transporter/SLC6A3 (6-8D6) valutazione 4.5 di 5 di 11.
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Schede tecniche

    • Anti-DAT/Dopamine Transporter/SLC6A3 Antibody 6-8D6 è un monoclonale di ratto IgG2a DAT/Dopamine Transporter/SLC6A3 antibody, citato in 10 pubblicazioni, fornito in 200 µg/ml
    • raised against amino acids 180-218 corresponding to the second extracellular loop of DAT of human origin
    • DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6) é raccomandato per il rilevamento di DAT di origine mouse, rat e human in WB, IP, IF e IHC(P)
    • DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di DAT/Dopamine Transporter/SLC6A3 (6-8D6): sc-32259.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6). This work is in progress.
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Are there reports using this DAT ab for Immunoprecipitation, if so which ones? Could it be used for magnetic isolation by coupling to Dynabeads?

Richiesta da: andresj1
Thank you for your question. DAT Antibody (6-8D6): sc-32259 has been tested and is recommended for IP applications with DAT of mouse, rat and human origin. As of now there are no publications in which this antibody has been used for immunoprecipitation.
Risposta da: Tech Support Europe
Data di pubblicazione: 2020-07-14

Can I use it on rat sections, blocking with normal donkey serum?

Richiesta da: ann1
Thank you for your question. Yes, this antibody is mouse, rat and human reactive, so using it on rat samples is fine. If you are doing a Western blot we would recommend blocking in 5% milk with TBS-T. You can block in normal donkey serum if you are doing an IHC or IF, especially if you are using a donkey anti-rat secondary antibody. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Risposta da: Tech Service
Data di pubblicazione: 2017-11-06

Is a blocking peptide for with this monoclonal antibody available?

Richiesta da: AbPolly
Thank you for your question. This DAT antibody sc-32259 is raised against a recombinant protein and not a short synthetic peptide and a blocking peptide is not possible to design for it.
Risposta da: Technical Support 7
Data di pubblicazione: 2017-05-16
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Rated 5 di 5 di da Works well for mouse brain tissue Purchased for IHC of mouse brain slices fixed in 0.4% PFA. Used 1:50 down to 1:4000 dilutions (in 4% BSA with 0.3% Triton-X100) and saw great staining of straitum.
Data di pubblicazione: 2021-07-15
Rated 4 di 5 di da great antibody I acquired the antibody a while ago. Works greatly and as good as other DATmAb from other vendors. We chose to purchase after sampling the product.
Data di pubblicazione: 2019-03-22
Rated 3 di 5 di da Good! I applied this antibody last year. This antibody couldn't use with rats. It's very sad.
Data di pubblicazione: 2018-03-02
Rated 5 di 5 di da 功能很出色 上半年申请的该抗体,用于westernblot实验,样品为N2A细胞,上样量为50 ug,曝光结果较好。
Data di pubblicazione: 2017-09-27
Rated 5 di 5 di da Clearness I used the antibody in WB at 1:300, PC12 cells. The protein band was clear, and there was no other bands. The pictures shows that the protein band is a little narrow, possibly because I used the 10% SDS separation gel.
Data di pubblicazione: 2017-09-18
Rated 5 di 5 di da this Adrenergic binding of the beta - adrenergic receptor activates adenylate cyclase, whereas adrenergic binding of the α2 - adrenergic receptor inhibits adenylate cyclase. The action of the molecule can be excitatory or inhibitory, depending on the type of receptor to which it is bound.
Data di pubblicazione: 2017-02-08
Rated 5 di 5 di da DAT (6-8D6) Antibody Works great for Western blot (WB), band at 80 kDa. We have not characterized the band above 90 kDa.
Data di pubblicazione: 2017-01-05
Rated 4 di 5 di da Pretty good... Used in IPs and WB and it worked as expected. Stable for years when stored correctly.
Data di pubblicazione: 2016-12-05
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