DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6): sc-32259

DAT Antibody (6-8D6) is a rat monoclonal IgG2a antibody that detects the DAT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-DAT antibody (6-8D6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The dopamine transporter (DAT) plays a crucial role in regulating dopamine levels in the synaptic cleft by facilitating the reuptake of dopamine from the extracellular space back into presynaptic neurons. This function is vital for maintaining dopamine homeostasis, which is essential for normal neurological function and behavior. Dysregulation of DAT activity has been implicated in several neuropsychiatric disorders, including attention deficit hyperactivity disorder (ADHD) and substance use disorders, highlighting DAT′s importance in both health and disease. DAT′s interaction with various neurotoxins underscores its significance in neurodegenerative conditions, particularly in the context of Parkinson′s disease, where DAT dysfunction can lead to the selective vulnerability of dopaminergic neurons.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6) References:

1. Role of dopamine transporter (DAT) in dopamine transport across the nasal mucosa.  |  Chemuturi, NV., et al. 2006. Life Sci. 79: 1391-8. PMID: 16733058
2. Dopamine transporter (DAT) inhibitors alleviate specific parkinsonian deficits in monkeys: association with DAT occupancy in vivo.  |  Madras, BK., et al. 2006. J Pharmacol Exp Ther. 319: 570-85. PMID: 16885433
3. Dopamine transporter (DAT) genotype (VNTR) and phenotype in extrapyramidal symptoms induced by antipsychotics.  |  Lafuente, A., et al. 2007. Schizophr Res. 90: 115-22. PMID: 17150335
4. Presence and function of dopamine transporter (DAT) in stallion sperm: dopamine modulates sperm motility and acrosomal integrity.  |  Urra, JA., et al. 2014. PLoS One. 9: e112834. PMID: 25402186
5. Dopamine transporter (DAT) genetic hypofunction in mice produces alterations consistent with ADHD but not schizophrenia or bipolar disorder.  |  Mereu, M., et al. 2017. Neuropharmacology. 121: 179-194. PMID: 28454982
6. In Vitro Assays for the Functional Characterization of the Dopamine Transporter (DAT).  |  Aggarwal, S. and Mortensen, OV. 2017. Curr Protoc Pharmacol. 79: 12.17.1-12.17.21. PMID: 29261226
7. Dopamine transporter (DAT) knockdown in the nucleus accumbens improves anxiety- and depression-related behaviors in adult mice.  |  Bahi, A. and Dreyer, JL. 2019. Behav Brain Res. 359: 104-115. PMID: 30367968
8. Altered Sexual Behavior in Dopamine Transporter (DAT) Knockout Male Rats: A Behavioral, Neurochemical and Intracerebral Microdialysis Study.  |  Sanna, F., et al. 2020. Front Behav Neurosci. 14: 58. PMID: 32372926
9. Allosteric Modulator KM822 Attenuates Behavioral Actions of Amphetamine in Caenorhabditis elegans through Interactions with the Dopamine Transporter DAT-1.  |  Refai, O., et al. 2022. Mol Pharmacol. 101: 123-131. PMID: 34906999
10. Missed Insights for Earlier Management of Parkinson's Disease and the Value of Dopamine Transporter (DAT) Scans.  |  Hafeez, M., et al. 2024. Geriatrics (Basel). 9: PMID: 39451858

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6) Specifications

Alternate Product Name: Dopamine Active Transporter (DAT)

Product Clone Name: 6-8D6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 180-218

Epitope Target: DAT

Epitope Species: human

Product Reactivity/Detection: DAT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred DAT/Dopamine Transporter/SLC6A3 antibody is DAT/Dopamine Transporter/SLC6A3 Antibody (6-5G10): sc-32258.