Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DACH1 CRISPR/Cas9 KO Plasmid (h): sc-404712
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DACH1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DACH1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DACH1 Antibody (A-6): sc-398706
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DACH1 CRISPR/Cas9 KO Plasmid (h) | sc-404712 | 20 µg | $397.00 |
Overview
DACH1 (dachshund family transcription factor 1) is a nuclear DNA-binding regulator implicated in cell fate decisions and tissue differentiation programs, with prominent roles in developmental signaling. In human cells, DACH1 modulates transcriptional networks linked to proliferation control, epithelial lineage maintenance, and cellular migration, including crosstalk with hormone receptor signaling and other transcription factor circuits. Altered DACH1 expression or regulatory activity has been associated with changes in tumor suppressive phenotypes, impacting processes such as cell-cycle progression, invasion, and metastasis-related gene expression. Its context-dependent function makes DACH1 a useful node for studying transcriptional repression/activation balance and pathway rewiring in disease-relevant models.
DACH1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DACH1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DACH1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DACH1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DACH1 protein expression.
This CRISPR knockout system enables efficient generation of DACH1-deficient cell models for investigation of DACH1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DACH1 exon(s) critical for DACH1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DACH1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DACH1 CRISPR/Cas9 KO Plasmid (h) and DACH1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DACH1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DACH1 HDR Plasmid (h) and DACH1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DACH1 homology arms to support homology-directed repair at defined DACH1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.