D1DR/Dopamine Receptor D1 Double Nickase Plasmid (h): sc-400532-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- D1DR/Dopamine Receptor D1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- D1DR/Dopamine Receptor D1 Double Nickase Plasmid (h) and D1DR/Dopamine Receptor D1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DRD1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: D1DR/Dopamine Receptor D1 Antibody (A-1): sc-518273
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
D1DR/Dopamine Receptor D1 Double Nickase Plasmid (h) | sc-400532-NIC | 20 µg | $410.00 | |||
D1DR/Dopamine Receptor D1 Double Nickase Plasmid (h2) | sc-400532-NIC-2 | 20 µg | $410.00 |
DRD1 encodes the dopamine receptor D1 (D1DR), a G protein–coupled receptor that primarily couples to Gαs/olf to stimulate adenylyl cyclase, elevate cAMP, and activate PKA-dependent signaling. D1DR signaling modulates neuronal excitability and synaptic plasticity through regulation of ion channels, CREB-mediated transcription, and cross-talk with MAPK/ERK pathways. In the central nervous system, DRD1 is a key component of dopaminergic neurotransmission within striatal and cortical circuits, influencing motor control, reward learning, and cognition. Dysregulated DRD1 signaling and receptor expression are frequently investigated in the context of neuropsychiatric and neurodegenerative disease mechanisms, including Parkinson’s disease and schizophrenia, as well as substance use–related adaptations.
D1DR/Dopamine Receptor D1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DRD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DRD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DRD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DRD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.