cyclin T1 Antibody (F-8): sc-515067

Cyclin T1 Antibody (F-8) is a mouse monoclonal IgG2a antibody that detects cyclin T1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cyclin T1 plays a crucial role in the regulation of transcription elongation by forming a complex with cyclin-dependent kinase 9 (Cdk9), which is essential for the phosphorylation of the C-terminal domain of RNA polymerase II. This phosphorylation is vital for the transition of RNA polymerase II from transcription initiation to elongation, thereby facilitating the production of mRNA. The interaction between cyclin T1 and the HIV-1 Tat protein is particularly significant, as cyclin T1 enhances Tat′s ability to bind to the viral TAR RNA, promoting the recruitment of the cyclin T1/Cdk9 complex to RNA polymerase II. This mechanism underscores cyclin T1′s importance not only in cellular transcription processes but also in viral replication, highlighting cyclin T1′s potential as a target for therapeutic interventions in diseases associated with dysregulated transcription. Anti-cyclin T1 antibody (F-8) is an invaluable tool for researchers studying transcriptional regulation and its implications in both normal physiology and disease states.

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cyclin T1 Antibody (F-8) References:

1. Human immunodeficiency virus type 1 infection induces cyclin T1 expression in macrophages.  |  Liou, LY., et al. 2004. J Virol. 78: 8114-9. PMID: 15254183
2. The role of the Cdk9/Cyclin T1 complex in T cell differentiation.  |  Leucci, E., et al. 2007. J Cell Physiol. 212: 411-5. PMID: 17352406
3. Recruitment of cyclin-dependent kinase 9 to nuclear compartments during cytomegalovirus late replication: importance of an interaction between viral pUL69 and cyclin T1.  |  Feichtinger, S., et al. 2011. J Gen Virol. 92: 1519-1531. PMID: 21450947
4. Cyclin K inhibits HIV-1 gene expression and replication by interfering with cyclin-dependent kinase 9 (CDK9)-cyclin T1 interaction in Nef-dependent manner.  |  Khan, SZ. and Mitra, D. 2011. J Biol Chem. 286: 22943-54. PMID: 21555514
5. HIV-1 replication and latency are regulated by translational control of cyclin T1.  |  Hoque, M., et al. 2011. J Mol Biol. 410: 917-32. PMID: 21763496
6. Cyclin T1 and CDK9 T-loop phosphorylation are downregulated during establishment of HIV-1 latency in primary resting memory CD4+ T cells.  |  Budhiraja, S., et al. 2013. J Virol. 87: 1211-20. PMID: 23152527
7. Computational study and peptide inhibitors design for the CDK9 - cyclin T1 complex.  |  Randjelović, J., et al. 2013. J Mol Model. 19: 1711-25. PMID: 23296566
8. A single point mutation in cyclin T1 eliminates binding to Hexim1, Cdk9 and RNA but not to AFF4 and enforces repression of HIV transcription.  |  Kuzmina, A., et al. 2014. Retrovirology. 11: 51. PMID: 24985467
9. Structure-guided design and cloning of peptide inhibitors targeting CDK9/cyclin T1 protein-protein interaction.  |  Taghizadeh, MS., et al. 2024. Front Pharmacol. 15: 1327820. PMID: 38808256
10. Discovery of a highly potent, selective, and stable d-amino acid-containing peptide inhibitor of CDK9/cyclin T1 interaction for the treatment of prostate cancer.  |  Xu, Z., et al. 2025. Eur J Med Chem. 285: 117248. PMID: 39808974

The preferred cyclin T1 antibody is cyclin T1 Antibody (E-3): sc-271348.