cyclin A Antibody (E67.1): sc-53230

Cyclin A Antibody (E67.1) is a mouse monoclonal IgG2a antibody that detects cyclin A in mouse, rat, human, and bovine samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Cyclin A plays a pivotal role in the regulation of the eukaryotic cell cycle, particularly during the S phase, where cyclin A is crucial for the proper progression and timing of DNA replication. Cyclin A is primarily located in the nucleus, where cyclin A associates with cyclin-dependent kinase-2 (Cdk2) to form active complexes that drive the cell cycle forward. Precise localization of cyclin A is essential, as cyclin A ensures that the cell cycle machinery is activated at the right time and place, preventing errors that could lead to genomic instability or uncontrolled cell proliferation. Cyclin A is also known to interact with various proteins, including the retinoblastoma protein and transcription factors such as E2F, highlighting cyclin A′s importance in cell cycle regulation and potential implications in cancer biology. Anti-cyclin A antibody (E67.1) is an invaluable tool for researchers studying cell cycle dynamics and the molecular mechanisms underlying cell proliferation and transformation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

cyclin A Antibody (E67.1) References:

1. beta3-endonexin as a novel inhibitor of cyclin A-associated kinase.  |  Ohtoshi, A., et al. 2000. Biochem Biophys Res Commun. 267: 947-52. PMID: 10673397
2. A complex degradation signal in Cyclin A required for G1 arrest, and a C-terminal region for mitosis.  |  Jacobs, HW., et al. 2001. EMBO J. 20: 2376-86. PMID: 11350926
3. Elevated levels of cyclin A1 and A (A2) mRNA in acute myeloid leukaemia are associated with increased survival.  |  Nakamaki, T., et al. 2003. Br J Haematol. 123: 72-80. PMID: 14510945
4. Cyclin A1 is a p53-induced gene that mediates apoptosis, G2/M arrest, and mitotic catastrophe in renal, ovarian, and lung carcinoma cells.  |  Rivera, A., et al. 2006. Cell Mol Life Sci. 63: 1425-39. PMID: 16799873
5. Altered expression of cyclin A 1 in muscle of patients with facioscapulohumeral muscle dystrophy (FSHD-1).  |  Pakula, A., et al. 2013. PLoS One. 8: e73573. PMID: 24019929
6. MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus.  |  Wang, S., et al. 2019. Cells. 8: PMID: 31390757
7. Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B.  |  Hégarat, N., et al. 2020. EMBO J. 39: e104419. PMID: 32350921
8. Cyclin A and Cks1 promote kinase consensus switching to non-proline-directed CDK1 phosphorylation.  |  Al-Rawi, A., et al. 2023. Cell Rep. 42: 112139. PMID: 36840943
9. Identification of a developmental timer regulating the stability of embryonic cyclin A and a new somatic A-type cyclin at gastrulation.  |  Howe, JA., et al. 1995. Genes Dev. 9: 1164-76. PMID: 7758942
10. A peptide corresponding to residues Asp177 to Asn208 of human cyclin A forms an alpha-helix.  |  Fan, JS., et al. 1998. Biochem Biophys Res Commun. 253: 621-7. PMID: 9918778

The preferred cyclin A antibody is cyclin A Antibody (BF683): sc-239.