CTRL CRISPR/Cas9 KO Plasmid (h): sc-410749
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CTRL CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CTRL genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTRL CRISPR/Cas9 KO Plasmid (h) | sc-410749 | 20 µg | $397.00 |
Overview
CTRL (chymotrypsin-like) encodes a serine protease that participates in proteolytic processing of dietary proteins and contributes to the broader digestive enzyme network. As a trypsin-like peptidase family member, CTRL activity aligns with extracellular proteolysis and can be considered within pathways governing protease activation, substrate turnover, and regulation by endogenous protease inhibitors. Variation in digestive protease expression or activity is frequently evaluated in studies of pancreatic and gastrointestinal biology, including mechanisms that influence enzyme secretion, zymogen activation, and mucosal proteostasis. These contexts make CTRL a useful molecular node for investigating how protease-dependent microenvironments affect cellular signaling and tissue physiology.
CTRL CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CTRL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CTRL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CTRL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CTRL protein expression.
This CRISPR knockout system enables efficient generation of CTRL-deficient cell models for investigation of CTRL signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CTRL exon(s) critical for CTRL function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CTRL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CTRL CRISPR/Cas9 KO Plasmid (h) and CTRL CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CTRL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CTRL HDR Plasmid (h) and CTRL HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CTRL homology arms to support homology-directed repair at defined CTRL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.