Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CSN8 CRISPR/Cas9 KO Plasmid (h): sc-409800
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CSN8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CSN8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CSN8 Antibody (F-8): sc-393482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CSN8 CRISPR/Cas9 KO Plasmid (h) | sc-409800 | 20 µg | $397.00 |
Overview
COPS8 encodes CSN8, a core subunit of the COP9 signalosome that regulates protein homeostasis by catalyzing deneddylation of cullin-RING ubiquitin ligases. Through this activity, CSN8 tunes ubiquitin-dependent turnover of key cell-cycle regulators, transcription factors, and stress-response proteins, influencing processes such as DNA damage signaling, autophagy–proteasome balance, and apoptosis. CSN8 function is integrated with broader ubiquitin–proteasome and neddylation pathways that control signal transduction and developmental programs. Dysregulation of COP9 signalosome components, including CSN8, has been associated with altered proliferative signaling and cellular stress adaptation relevant to cancer biology and neurodegenerative phenotypes.
CSN8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COPS8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COPS8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COPS8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CSN8 protein expression.
This CRISPR knockout system enables efficient generation of COPS8-deficient cell models for investigation of CSN8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COPS8 exon(s) critical for CSN8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COPS8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CSN8 CRISPR/Cas9 KO Plasmid (h) and CSN8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COPS8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CSN8 HDR Plasmid (h) and CSN8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COPS8 homology arms to support homology-directed repair at defined COPS8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.