Cryptic CRISPR/Cas9 KO Plasmid (m): sc-419633
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cryptic CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cryptic genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cryptic CRISPR/Cas9 KO Plasmid (m) | sc-419633 | 20 µg | $397.00 |
Overview
Mouse Cfc1 encodes Cryptic, a GPI-anchored co-receptor in the EGF-CFC family that modulates NODAL signaling during early embryogenesis. Cryptic supports SMAD2/3-dependent transcriptional programs that govern left–right axis specification, gastrulation movements, and mesendoderm patterning. Disruption of Cfc1 perturbs TGF-β superfamily pathway dynamics and is linked to laterality defects and congenital heart malformations in developmental genetics studies. As a surface-associated signaling regulator, Cryptic provides a tractable entry point for probing ligand–receptor complex assembly and context-specific NODAL responsiveness.
Cryptic CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cfc1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cfc1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cfc1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cryptic protein expression.
This CRISPR knockout system enables efficient generation of Cfc1-deficient cell models for investigation of Cryptic signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cfc1 exon(s) critical for Cryptic function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cfc1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cryptic CRISPR/Cas9 KO Plasmid (m) and Cryptic CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cfc1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cryptic HDR Plasmid (m) and Cryptic HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cfc1 homology arms to support homology-directed repair at defined Cfc1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.