Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CRX Double Nickase Plasmid (h): sc-401736-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRX Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CRX Double Nickase Plasmid (h) and CRX Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CRX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRX Antibody (A-9): sc-377138
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRX Double Nickase Plasmid (h) | sc-401736-NIC | 20 µg | $410.00 | |||
CRX Double Nickase Plasmid (h2) | sc-401736-NIC-2 | 20 µg | $410.00 |
CRX (cone-rod homeobox) encodes a retina-specific homeodomain transcription factor that is essential for photoreceptor differentiation and maintenance. In the nucleus, CRX coordinates gene regulatory programs controlling phototransduction components, outer segment biogenesis, and synaptic maturation by binding cis-regulatory elements and cooperating with other retinal transcription factors. It modulates transcriptional networks linked to cGMP signaling, opsin expression, and metabolic support required for cone and rod function. Altered CRX activity or expression is associated with inherited retinal degeneration phenotypes, making it a key target for mechanistic studies of photoreceptor development and disease-relevant gene regulation.
CRX Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CRX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CRX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CRX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CRX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.