Anticorpo CRMP-4 (LL-5) raccomandato per il rilevamento di CRMP-4 di origine mouse, rat e human in WB, IP e ELISA

Anticorpo CRMP-4 (LL-5): sc-100323

Anticorpo CRMP-4 (LL-5) valutazione 4.2 di 5 di 5.
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Schede tecniche

    • Anti-CRMP-4 Antibody LL-5 è un monoclonale di topo IgG2a (kappa light chain) CRMP-4 antibody fornito in 100 µg/ml
    • generato contro il ricombinante CRMP-4 di origine human
    • raccomandato per il rilevamento di CRMP-4 di origine mouse, rat e human in WB, IP e ELISA
    • Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.
    • Contattaci per ricevere un FREE 10 µg sample di CRMP-4 (LL-5): sc-100323.

This antibody has worked well for us for Western Blot. I now want to test CRMP4 levels by immunofluorescence in mouse brain slices. This antibody is only recommended for WB, is there any chance it would work for IF? Or would that be a waste of time?

Richiesta da: OCC1
Thank you for your question. We have not yet tested this antibody for IF or IHC in tissues or cells, and so this is not a recommended application at this time. However, it certainly may be worth trying since you already have the antibody. The two citations we are aware of currently are using the antibody in Western blot and IP/WB.
Risposta da: Technical Support
Data di pubblicazione: 2019-02-25

我想咨询这一款抗体(编号sc-100323)具体识别CRMP4蛋白(共计570个氨基酸)哪一段序列?谢谢!

Richiesta da: Chutian
感谢您的提问。CRMP-4 Antibody (LL-5): sc-100323免疫原是人源重组CRMP-4,我们没有检测过其具体识别哪一段序列。Thank you for your question. CRMP-4 Antibody (LL-5): sc-100323 is raised against recombinant CRMP-4 of human origin, we do not know which specific sequences this antibody would recognize.
Risposta da: Technical Support 8
Data di pubblicazione: 2018-12-21

For Western Blot, is it recommended to use denatured or non-denatured conditions with CRMP-4 (LL-5): sc-100323 antibody?

Richiesta da: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Risposta da: Technical Support
Data di pubblicazione: 2017-03-27
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Rated 5 di 5 di da Good specificity Gave a single specific band for endogenous U2OS cell lysate and no band when knocked down using RNAi as expected.
Data di pubblicazione: 2018-03-08
Rated 4 di 5 di da Strong band in WB in mouse transfected 293T and SK Strong band in WB in mouse transfected 293T and SK-N-MC whole cell lysates. -SCBT QC
Data di pubblicazione: 2015-05-11
Rated 4 di 5 di da Positive signal in WB in Jurkat whole cell lysate Positive signal in WB in Jurkat whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-04-19
Rated 4 di 5 di da Good specificity in WB in human transfected Good specificity in WB in human transfected 293T whole cell lysates. -SCBT QC
Data di pubblicazione: 2015-01-29
Rated 4 di 5 di da Robust signal in WB in human transfected Robust signal in WB in human transfected 293T whole cell lysates and rat brain tissue extract. -SCBT QC
Data di pubblicazione: 2014-02-25
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