Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CRIK Double Nickase Plasmid (h): sc-417835-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRIK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CRIK Double Nickase Plasmid (h) and CRIK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CIT. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRIK Antibody (E-6): sc-390437
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRIK Double Nickase Plasmid (h) | sc-417835-NIC | 20 µg | $410.00 | |||
CRIK Double Nickase Plasmid (h2) | sc-417835-NIC-2 | 20 µg | $410.00 |
Citron kinase (CIT; CRIK) is a RhoA-associated serine/threonine kinase that localizes to the cleavage furrow and midbody to coordinate late-stage cytokinesis. It supports contractile ring organization and abscission by regulating actin–myosin dynamics and cytoskeletal remodeling, linking Rho GTPase signaling to cell-cycle progression. Disruption of CIT function can lead to cytokinesis failure, multinucleation, and genome instability, processes that intersect with mitotic stress responses and tumor biology. In addition, CIT has been studied in the context of proliferative tissues and neurodevelopmental programs where accurate cell division is essential.
CRIK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CIT locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CIT. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CIT function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CIT-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.